toxicological in vitro tests

toxicological in vitro tests

We perform the in vitro tests of cytotoxicity and skin irritation potential of chemicals and products.

 

in vitro testing of skin irritation

Skin irritation refers to the production of reversible damage to the skin following the application of a test chemical for up to 4 hours [as defined by the United Nations (UN) Globally Harmonized System of Classification and Labelling of Chemicals (GHS)]. We perform the in vitro testing that may be used for the hazard identification of irritant chemicals (substances and mixtures) in accordance with UN GHS and EU CLP Category 2. Test are made on the three dimensional skin model EpiDerm SIT (EPI-200), Mattek, and may be used to determine the skin irritancy of chemicals as a stand-alone replacement test for in vivo skin irritation testing on rabbits.


Testing is performed in accordance with the following guidelines:

 

  • OECD Guidelines for testing of chemicals: TG 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method), 6/2015
  • In Vitro EpiDerm Skin Irritation Test (EPI-200-SIT), 11/2014, Mattek

 

The test is applicable to solids, liquids, semi-solids and waxes. Gases and aerosols have not been assessed yet in a validation study.

 

 

in vitro testing of skin corrosion

Skin corrosion refers to the production of irreversible tissue damage in the skin following the application of a test material, as defined by the Globally Harmonized System (GHS). We perform the in vitro testing on the three dimensional skin model EpiDerm SCT (EPI-200), Mattek. This test is able to reliably discriminate chemicals that are corrosive to skin from non-corrosive chemicals, and therefore be used for the classification of skin corrosion hazard according to the GHS system adopted by the OECD. The test is not designed to distinguish between sub-categories of corrosivity.

 

Testing is performed in accordance with the following guidelines:

  • OECD Guidelines for testing of chemicals: TG 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis Test Method), 6/2015
  • In Vitro EpiDerm Skin Corrosion Test (EPI-200-SCT), 11/2014, Mattek

 

 

in vitro testing of cytotoxicity

We provide laboratory testing for cytotoxicity potential of medical devices, chemicals, cosmetic products, personal protective equipment and toys.


The testing has been performed on cultured cells monolayers according following legislation:

  • ISO 10993-5: Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity